What is 1M DTT?
DTT is an essential component for kinase assays using LanthaScreen® Technology. Invitrogen’s DTT is 1M in water. It is tested for background fluorescence, making it optimal for use in high-throughput screening applications.
How do you dilute DTT?
To prepare a 1 M DTT solution, dissolve 1.55 g of DTT powder in 10 mL of deionized water (e.g. Water, nuclease-free, #R0581).
How do you make 100mM DTT solution?
(1) Prepare a fresh solution of 100mM DTT (15.4mg in 1 mL water). Add __μL (to be calculated per sample=Sample vol /20) of DTT solution to each sample, this will give final concentration of 5 mM. Vortex and incubate at 60°C for 30min. This should remove disulphide bonds in proteins.
How long is DTT good in solution?
Storage and Stability A solution of DTT in Hepes buffer (pH 7.75) is stable for one week at +2 to +8°C if the container is tightly sealed and the solution is pro- tected from atmospheric oxygen by argon or nitrogen.
How do you make a 2m DTT solution?
Prepare 800 mL of distilled water in a suitable container. Add 150 g of DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) to the solution. Add distilled water until the volume is 1 L. Dispense into 1-mL aliquots, and store them in the dark (wrapped in aluminum foil) at -20°C (indefinitely).
How do you make a 1m stock solution?
Molarity (M) means the number of moles of solute per liter of solution. To prepare a 1 M solution, slowly add 1 formula weight of compound to a clean 1-L volumetric flask half filled with distilled or deionized water. Allow the compound to dissolve completely, swirling the flask gently if necessary.
How do I make 1M of DTT?
How to make a 1M DTT Stock Solution
- Weigh 1.54 g of Dithiothreitol (DTT) 2 g.
- Add 10 ml of sterile dH2O. Dissolve completely.
- Prewet a 0.2 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water. 00:02:00.
- Sterilize DTT Stock through the prepared 0.2 µm syringe filter.
How do you make a 1M stock solution?
How fast does DTT degrade?
Yes, DTT demonstrates degradation starting after 3 days and increasing rapidly after 5 days at 30°C.
How do you calculate how much stock solution you need?
To determine the volume of stock solution needed, divide the number of moles of glucose by the molarity of the stock solution.
How do you make 50 mm DTT?
Add 7.7 mg of DTT (FW 154) to a 1.5-mL microcentrifuge tube. Add 1 mL of Milli-Q H2O. Prepare fresh.
Is DTT soluble in water?
Preparation Instructions This product is soluble in water (50 mg/ml), yielding a clear, colorless solution. DTT is also soluble in ethanol, acetone, ethylate, chloroform, and ether.
Is DTT stable in water?
Freely soluble in water, ethanol, acetone, ethylate, chloroform and ether. It forms a clear, colorless solution at 50 mg/mL in water. DTT has a lower volatility than that of other reducing agents such as b-mercaptoethanol. DTT is not stable in solution, thus only freshly-made DTT solutions should be used.
How do you make 10 mm solution from 1M?
Take 1 part of your stock solution and add 9 parts of solvent (usually water but sometimes alcohol or other organic solvent). In all cases you are diluting by the same factor. The concentration of the resulting solution is 1M /10 = 0.1M where 10 is the dilution factor.
How do you make a 1 m DTT solution?
Only freshly-made DTT solutions should be used. To prepare a 1 M DTT solution, dissolve 1.55 g of DTT powder in 10 mL of deionized water (e.g. Water, nuclease-free, #R0581).
What is the composition of DTT in a reducing agent?
DTT (Dithiothreitol) is a commonly used reducing agent. Table 1. Required components Prepare 800 mL of distilled water in a suitable container. Add 150 g of DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) to the solution.
What is the concentration of DTT in SDS?
Description. At a final 0.1 M concentration, DTT is also widely used for disruption of protein disulfide bonds in SDS-polyacrylamide gel electrophoresis. • Free of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases. Note: DTT is not stable in solution. Only freshly-made DTT solutions should be used.
What is the best way to prepare DTT?
DTT (Dithiothreitol) is a commonly used reducing agent. Table 1. Required components Prepare 800 mL of distilled water in a suitable container. Add 150 g of DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) to the solution. Dispense into 1-mL aliquots, and store them in the dark (wrapped in aluminum foil) at -20°C (indefinitely).