What wavelength is RFP?
Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
At what wavelength does RFP fluorescence?
RFP (TagRFP) is a fluorescent compound with an excitation peak at 555 nm and an emission peak at 583 nm.
Are RFP and mCherry the same?
mCherry is a member of the mFruits family of monomeric red fluorescent proteins (mRFPs). As a RFP, mCherry was derived from DsRed of Discosoma sea anemones unlike green fluorescent proteins (GFPs) which are often derived from Aequoera victoria jellyfish.
How much kDa is GFP?
27 kDa
The green fluorescent protein (GFP), originally discovered in the jellyfish, Aequorea victoria, is composed of 238 amino acids and has a molecular weight of 27 kDa.
What makes GFP RFP fluoresce when exposed UV light?
Scientists knew that GFP glows because three of its amino acids form a fluorophore, a chemical group that absorbs and emits light.
What is mCherry used for?
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments.
What Colour is RFP?
Red Fluorescent Protein (RFP)
What is the molecular weight of RFP?
Red Fluorescent Protein (RFP) Protein Protein RFP is suitable as a control for polyclonal or monoclonal anti-RFP in immunological assays. The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa.
What is the MW of GFP?
27 kD
One interesting candidate gene that fulfills these requirements is the gene-encoding green fluorescent protein (GFP). It was originally isolated from the jellyfish Aquorea victoria. The GFP cDNA consists of 730 bp, which encode a 238 amino acid protein with a molecular weight of 27 kD (2).
Does RFP antibody recognize mCherry?
The anti-RFP antibody 5F8 recognizes common RFP variants including mCherry, mPlum, mRFP, mRFPruby.