Which catalyst is used for capping in solid-phase DNA synthesis?
Step 3: Capping The capping step is performed by treating the solid support-bound material with a mixture of acetic anhydride and 1-methylimidazole or, less often, DMAP as catalysts and, in the phosphoramidite method, serves two purposes.
What is chemical DNA synthesis?
DNA synthesis is the natural or artificial creation of deoxyribonucleic acid (DNA) molecules. DNA is a macromolecule made up of nucleotide units, which are linked by covalent bonds and hydrogen bonds, in a repeating structure.
How are DNA oligos synthesized?
Custom DNA oligos are made by a process called synthesis or more specifically, solid-phase chemical synthesis. This is a method in which the 4 nucleic acids, A, T, C, and G, are added one by one to form a growing chain of nucleotides. They are built on an oligo building block called a phosphoramidite.
What is the meaning of solid phase?
Noun. solid phase (plural solid phases) (physics) One of the three fundamental structural phases of matter in which the cohesive force of matter is strong enough to keep the molecules or atoms in the given positions, restraining the thermal mobility.
Is DNA replicated in the S phase?
The S phase of a cell cycle occurs during interphase, before mitosis or meiosis, and is responsible for the synthesis or replication of DNA.
How is synthetic DNA synthesized?
(From top, clockwise) Synthetic DNA constructs are designed and manipulated using computer-aided design software. The designed DNA is then divided into synthesizable pieces (synthons) up to 1–1.5 kbp. The synthons are then broken up into overlapping single-stranded oligonucleotide sequences and chemically synthesized.
Why is solid phase synthesis?
General advantages of solid phase synthesis are easy purification, rapid generation of linear peptide intermediates, and precedent in the synthesis of large peptides. Given the ease with which peptide linear precursors are produced on solid phase, we opted to employ this approach in addition to a solution phase method.
Why do we use solid phase extraction?
Solid phase extraction prevents most problems encountered with liquid-liquid extraction and improves quantitative recovery yields. This technique is rapid (most extraction in less than 30 min), easy to perform and can be automated.
What is DMT in DNA?
In the current work we discovered that acid-labile 4,4′-dimethoxytrityl protecting group (DMT) of oligonucleotides (ONs) is stable under PCR conditions and does not interfere with activity of DNA polymerases.
Is DMT hydrophobic?
This purification can be achieved by using hydrophobic interaction chro- matography (HIC) since the DMT-on group is strongly hydrophobic.
Why lagging strand synthesis is discontinuous?
On the upper lagging strand, synthesis is discontinuous, since new RNA primers must be added as opening of the replication fork continues to expose new template. This produces a series of disconnected Okazaki fragments.
Why is DNA synthesis in S phase necessary?
S phase, or synthesis, is the phase of the cell cycle when DNA packaged into chromosomes is replicated. This event is an essential aspect of the cell cycle because replication allows for each cell created by cell division to have the same genetic make-up.