Are dengue proteins?
The dengue virus is a roughly spherical structure composed of the viral genome and capsid proteins surrounded by an envelope and a shell of proteins. After infecting a host cell, the dengue virus hijacks the host cell’s machinery to replicate the viral RNA genome and viral proteins.
Which viral protein can be identified by Elisa in dengue?
Capture ELISA for detection of the NS1 protein. After wells were washed as described above, human sera or purified dengue virus type 1 NS1 diluted in PBS-Tween buffer was added to wells (40 μl/well) and incubated for 2 h at room temperature.
What is the structure of dengue virus?
The dengue virus has a roughly spherical structure. It is composed of the viral genome and capsid proteins surrounded by an envelope and a shell of proteins. After infecting a host cell, the dengue virus hijacks the host cell’s machinery to replicate the viral RNA genome and viral proteins.
What is non structural protein 1 dengue?
Nonstructural protein 1 (NS1) is a multifunctional protein actively secreted in vertebrate and mosquito cells during infection. In mosquito cells, NS1 is secreted in a caveolin-1-dependent manner by an unconventional route.
What protein enables the flu virus to get out of a cell?
Influenza A virus assembles at the plasma membrane of infected host cells, releases by budding, and possesses a lipid membrane derived from the host cell (1). This envelope contains two important transmembrane glycoproteins: hemagglutinin (HA) and neuraminidase (NA).
What is dengue IgM and IgG?
Positive IgM and IgG tests for dengue antibodies detected in an initial blood sample mean that it is likely that the person became infected with dengue virus within recent weeks. IgM antibody tests can be positive if a person has been infected with a similar virus, such as chikungunya (called cross-reaction).
What is the genetic material of dengue virus?
Genomic Structure of Dengue Virus. The DENV genome consists of a single, positive-strand RNA of ~11 kb. A type I cap is present at the 5′ end, followed by the conserved dinucleotide 5′-AG-3′ (Cleaves and Dubin, 1979).
What is serotype of dengue?
Dengue is caused by a virus of the Flaviviridae family and there are four distinct, but closely related, serotypes of the virus that cause dengue (DENV-1, DENV-2, DENV-3 and DENV-4). Recovery from infection is believed to provide lifelong immunity against that serotype.
What is unique about the dengue virus?
Dengue has distinct epidemiological patterns, associated with the four serotypes of the virus. These can co-circulate within a region, and indeed many countries are hyper-endemic for all four serotypes. Dengue has an alarming impact on both human health and the global and national economies.
How many structural and nonstructural protein are there in the dengue virus?
The 11kb RNA genome of the dengue virus encodes three structural proteins (envelope, pre-membrane, capsid) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5), all of which are translated as a single polyprotein that is subsequently cleaved by viral and host cellular proteases at specific …
Why are viruses proteins important?
These viral proteins control and influence viral gene expressions in the viral genome, including viral structural gene transcription rates. Viral regulatory and accessory proteins also influence and adjust cellular functions of the host cell, such as the regulation of genes, and apoptosis.