What are the functions of the WBC diluting fluid?
WBC diluting fluid is used for perfoming the WBC (Leucocyte) count. Glacial acetic acid lyses the red cells. Gentian violet slightly stains the nuclei of the leucocytes.
What is the purpose of the bead present in the bulb of the WBC pipette?
The functions of The White Bead present in WBC Pipette are: > This white bead which is present in the bulb helps in the identification of the pipette. > It also helps in the mixing of diluting fluid and blood.
What is the dilution factor in the WBC pipette?
0.5 part of blood is mixed in 10 parts of fluid So, 1 part of blood is in 20 parts of fluid Thus, dilution factor for WBC counting is 20.
What is dilution used in RBC pipette?
The RBC pipette is the diluting pipette used in dilute blood ratio of ‘1:100’ or ‘1:200’ in the Hemocytometer. It uses the RBC pipette as a liquid dispenser. It has been used in chemistry, biology & medicine as a laboratory instrument.
Why is the type of cell diluting fluid important?
Why is the type of cell diluting fluid important? RBC diluting fluid must be isotonic to prevent red cell lysis, while WBC diluting fluid must destroy the red cells in order for the WBCs to be visible.
Which pipette is used for platelet count?
WBC pipette is a graduated pipette that gives the dilution of 1:20. It has two markings at the bottom as 0.5 and 1 and the top of the pipette is marked 11. It has a round shape bulb which contains the White bead to mix the blood specimen and the diluting fluid.
Which diluting fluid is used for WBC count?
Normal saline should be used to make dilutions for total cell counts. Diluting fluids for WBC counts include: crystal violet/acetic acid. gentian violet/acetic acid.
What are the diluting fluids used for WBC count and RBC count?
The diluting fluid for RBCs is isotonic saline. Diluting fluids for WBCs include glacial acetic acid to lyse the RBCs, or Türk solution, which contains glacial acetic acid and methylene blue to stain the nuclei of the WBCs.
Why is blood diluted?
Dilution with physiologic saline solution and other fluids accelerates the coagulation of properly collected normal and hemophilic blood and plasma in silicone coated vessels, with or without the aid of activating agents such as platelets, thromboplastin, cephalin, glass particles or plasma euglobulin fractions.
What dilution is used for WBC count?
The standard dilution of blood for leukocyte counts is 1:20; therefore the dilution factor is 20. The volume of diluted blood used is based on the area and depth of the counting area. The area counted is 4 mm2 and the depth is 0.1 mm; therefore the volume factor is 0.4 mm3.
What is the volume of WBC pipette?
Glass WBC Pipette, Capacity: 1 ml
| Capacity | 1 ml |
|---|---|
| Material | Glass |
| Color | Transparent |
| Country of Origin | Made in India |
| Mfg By | Recombigen Clear & Sure |
Can the RBC pipette be used for WBC count?
It is used for counting WBC in leukemia where leukocytes are present not in thousands but in lakhs or millions/mm3 of blood. There also blood is sucked upto mark 1 in RBC pipette and then diluted to 101 mark giving a dilution of 1 in 100. The RBC pipette is also used for sperm count and platelet count.
How do you identify a RBC and WBC pipette?
The RBC pipette is identified by the red bead in the bulb, and the WBC pipette by the white bead in the bulb.
Why do we perform manual WBC count?
A hemocytometer is charged with the diluted blood and nuclei are counted in the appropriate areas of the grid using a light microscope. The manual WBC is a reasonable test to do in-office since it is not terribly time- and labor-intensive and gives acceptably accurate results.
What diluting fluid is most commonly used when performing WBC count?
Diluting Fluid used for WBC count. WBC diluting fluid is used for performing the WBC (Leucocyte) count. Glacial acetic acid lyses the red cells. Gentian violet slightly stains the nuclei of the leucocytes.
What is WBC pipette?
WBC Pipette or White Blood Cell Pipette is used for mixing diluting fluid and blood. This pipette is used in higher dilution processes, such as higher total sperm count, platelet count, total leukocyte count, RBC count in severe anaemia, etc.
What is manual WBC count?
A manual WBC differential count is performed by having a person trained in peripheral blood morphology review the stained blood smear and manually count 100 white cells (or 50 cells in the case of severe leukopenia).