What is RNA splicing PPT?
RNA Splicing Introduction In molecular biology and genetics, splicing is a modification of the nascent pre-messenger RNA (pre-mRNA) transcript in which introns are removed and exons are joined. For nuclear encoded genes, splicing takes place within the nucleus after or concurrently with transcription.
What is alternative splicing PPT?
ALTERNATIVE SPLICING • It is deviated from constitutive splicing which is a regulated process during gene expression that results in a single gene coding for multiple proteins wherein, particular exons maybe included or excluded from final, processed mRNA.
How are introns removed?
Introns are removed from primary transcripts by cleavage at conserved sequences called splice sites. These sites are found at the 5′ and 3′ ends of introns. Most commonly, the RNA sequence that is removed begins with the dinucleotide GU at its 5′ end, and ends with AG at its 3′ end.
What are advantages of introns?
But introns may also benefit their hosts. Evolutionary advantages of introns include the possibility to create new genes by cutting and pasting exons from existing genes or to diversify the protein output of a single gene by splicing the exons together in different ways.
What is the purpose of splicing?
Splicing makes genes more “modular,” allowing new combinations of exons to be created during evolution. Furthermore, new exons can be inserted into old introns, creating new proteins without disrupting the function of the old gene.
What is the importance of introns?
The most important function of introns is that they allow for alternative splicing, making it possible to generate multiple proteins from a single gene. Some introns encode functional RNA molecules through further processing after they are spliced.
What is the importance of intron?
Introns are crucial because the protein repertoire or variety is greatly enhanced by alternative splicing in which introns take partly important roles. Alternative splicing is a controlled molecular mechanism producing multiple variant proteins from a single gene in a eukaryotic cell.
How are introns spliced out?