What is usp L1 column?
According to the United States Pharmacopeia (USP), L1 column packing is defined as “octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 3 to 10 m in diameter.” Some USP methods specifying an L1 column use intermediate pH, and many of these methods were done on a 10 m, 3.9 x 300 mm column.
What are HPLC columns used for?
Size exclusion HPLC columns are used primarily for the separation of proteins and carbohydrates. Other types of HPLC columns include affinity, ion exclusion, and displacement chromatography columns; a chiral HPLC column can be used to resolve racemic mixtures.
How many types of HPLC columns are there?
High performance liquid chromatography (HPLC) is a type of liquid chromatography that uses a liquid moblie phase. The same basic principals from gas chromatography are applied to liquid chromatography. There are three basic types of liquid chromatographic columns: liquid-liquid, liquid-solid, and ion-exchange.
How much are HPLC columns?
The cost of HPLC columns can range anywhere between US $ 500 – 1000 depending on the specificity of applications.
What is the range of HPLC?
In this study, the HPLC hemoglobin reference ranges derived from 200 normal African American adults are expressed as follows: Hb A mean 93.6 percent (s.d. 1.3, ranges 89.8 to 95.2), Hb A1 mean 2.0 percent (s.d. 0.6, ranges 0.8 to 5.2), Hb F mean 3.2 percent (s.d. 0.7, ranges 1.7 to 5.3) and Hb A2 mean 1.2 percent (s.d. …
What is the cost of LC MS?
Fixed Liquid Chromatography Mass Spectrometer, Rs 1500000 | ID: 3937862655.
What is the cost of HPLC in India?
The cost of HPLC Hemoglobin Electrophoresis in India varies from ₹ 450 to ₹ 800 in 21 cities of India. The lowest price of HPLC Hemoglobin Electrophoresis, amongst the metro cities, is at Delhi. HPLC Hemoglobin Electrophoresis Costs ₹ 450 in Delhi. The market price is much higher at ₹ 1000.
What is C4 column?
YMC-Pack C4 (Butyl) columns are less hydrophobic than C8 or C18 packings, and generally utilize more aqueous eluents than either of these reversed phase column types. When compared to C8 and C18 columns using the same eluent, C4 columns show significantly shorter retention for non-polar compounds.
What is detector in HPLC?
HPLC detectors are used in the detection of the solute present in the eluent coming from the HPLC column. They are capable of determining the identity and concentration of eluting compounds in the mobile phase.
How many detectors are there in HPLC?
Fluorescence HPLC Detectors There are three types of fluorescent detectors, namely the single wavelength fluorescent detector, multi-wavelength fluorescent detector and laser-induced fluorescent detector.
What is ODS in HPLC columns?
Octadecyl-silica (ODS) is the industry standard packing material for HPLC applications. YMC offers packing materials with an impressive assortment of functional groups for liquid chromatography and a selection of ODS columns that is far ahead of all others in variety.
What is difference between ODS and C18 column?
The AQ type C18 column, such the ODS-B, has an end-capping that reduces phase collapse greatly, so it can be run in 100% water if needed. The ODS-A column has a more typical hydrophobic end-capping.
What is an ultra 1 GC column?
Ultra 1 GC column | Agilent Ultra-1 Columns Agilent J&W Ultra 1 is a 100% dimethylpolysiloxane, nonpolar column that resembles HP-1 but has tighter specifications for retention index and capacity factors. The column has an operating temperature range of –60 to 325/350 °C and is intended for use with the Sadtler Retention Index Library.
What is a restek ultra column?
Consult a Technical Specialist The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support. Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, these columns are designed for selective and reliable HPLC applications.
What is the Ultra line?
The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support. Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, these columns are designed for selective and reliable HPLC applications.
Why choose Agilent J&W hp-1ms ultra inert GC columns?
Tested with the tightest industry QC specifications for column bleed, sensitivity, and efficiency Application note for Chlorinated Solvents and Disinfection By-Product Analysis Using Agilent J&W HP-1ms Ultra Inert and DB-1301 Capillary GC Columns