What is animal cell culture and its application?
Animal cell cultures are used to replicate the viruses instead of animals for the production of vaccine. Cell culture can also be used to detect and isolate viruses, and also to study growth and development cycle of viruses. It is also used to study the mode of infection.
What are the four major instruments used in animal cell culture and write its uses?
Basic Equipment
- Cell culture hood (i.e., laminar-flow hood or biosafety cabinet)
- Incubator (humid CO2 incubator recommended)
- Water bath.
- Centrifuge.
- Refrigerator and freezer (–20°C)
- Cell counter (e.g., Countess® II FL Automated Cell Counter or hemacytometer)
- Inverted microscope.
What is culture media in animal cell culture?
A typical culture medium is composed of a complement of amino acids, vitamins, inorganic salts, glucose and serum as a source of growth factors, hormones and attachment factors. In addition to nutrients, the medium also helps maintain pH and osmolality.
Who is the father of animal cell culture?
The development of animal tissue culture commenced after the breakthrough frog tissue culture technique, which was discovered by Harrison in 1907. Due to this effort Harrison is considered as the father of tissue culture.
What materials and equipments are used in animal cell culture?
List of Basic Equipments needed in animal cell culture lab:
- Sterile Work Area/Cell culture hood (i.e., laminar-flow hood or biosafety cabinet)
- Incubator (humid CO2 incubator recommended)
- Water bath.
- Centrifuge.
- Refrigerator and freezer (–20°C)
- Cell counter (e.g. Automated Cell Counter or hemocytometer)
- Inverted microscope.
How many types of culture media are there?
These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media.
What are the types of culture media for animal cell culture?
Body fluid such as plasma, serum, lymph, amniotic fluid and much more are used. These fluids used as animal cell culture media after testing for toxicity and sterility. 2. Tissue extract such as extract of liver, spleen, bone marrow and leucocyes also used as animal cell culture media.
What are the techniques of animal cell culture?
Primary Cell Culture. It is defined as the cell culturing from the tissue of the host animal.
What are the disadvantages of animal cell culture?
Cell culture techniques are usually capital intensive.
What are characteristics of an animal cell?
Characteristics of the Animal Kingdom. The animal kingdom is very diverse,but animals share many common characteristics,such as methods of development and reproduction.
What are examples of animal cells?
Cell (Plasma) Membrane – thin,semi-permeable membrane that surrounds the cytoplasm of a cell,enclosing its contents.