What do anti-GFP antibodies do?
Anti-GFP antibody (ab290) is a highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin.
Which antibody is tagged with GFP?
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms.
Can GFP detect antibodies?
Anti-GFP antibodies are available as polyclonal or monoclonal antibodies and suited for detection of GFP, GFP variants and GFP fusion proteins on western blots, immunoprecipitation, immunohistochemistry, immunocytochemical localization and immunosorbent assays (ELISA).
Does GFP antibody recognize zsGreen?
See link below. Although Green Fluorescent, GFP and zsGreen share NO homology, therefore GFP antibodies wont work.
Can GFP antibody detect CFP?
Q: What GFP variants will this antibody recognize? A: Our anti-GFP antibody is a mouse monoclonal raised against the N-terminal of GFP and will recognize native and denatured GFP and its variants, including EGFP, YFP, EYFP, and CFP.
Can GFP antibody detect EGFP?
The Full-Length GFP Polyclonal Antibody detects AcGFP1, wild type GFP, and GFPuv. Based on their homology to GFP, we also expect this antibody to detect EGFP, EYFP, and ECFP.
What is Zsgreen?
ZsGreen1 is the brightest commercially available green fluorescent protein—up to 4X brighter than EGFP—and is ideally suited for whole-cell labelling, promoter-reporter studies, or as a transfection control. Living Colors ZsGreen1 is an exceptionally bright green fluorescent protein derived from a Zoanthus sp.
Does anti GFP antibody recognize YFP?
Affinity purified Anti-GFP (Green Fluorescent Protein) Tag clone GF28R, mouse IgG2b. Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP.
Do GFP antibodies recognize EGFP?
What is RFP and GFP?
What are RFP and GFP Antibodies? Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP) are ~29 kDa fluorescent proteins commonly used as markers for gene expression and protein localization. They can also be used to monitor physiological processes or detect transgenic or transient protein expression.
What is the difference between EGFP and GFP?
The main difference between GFP and EGFP is that the GFP (stands for Green Fluorescent Protein) is a protein that exhibits bright green fluorescence when exposed to blue light whereas the EGFP (stands for Enhanced Green Fluorescence Protein) exhibits stronger fluorescence than GFP.
What does the RFP gene do?
Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo.
What is GFP used for in microbiology?
GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
Why is GFP used as a reporter protein?
GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted.
What is the ELISA and immunoprecipitation of Aequorea victoria?
ELISA: 1:200-1:2,000, Immunocytochemistry: 1:50, Immunoprecipitation: 1:200-1:2,000, Western Blot: 2 µg/mL The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal.
What can GFP technology tell us about the physiology of cells?
As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells.