What is the kDa ladder?
Prestained Protein Ladder, Broad Range (10-230 kDa) is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. The protein concentrations are carefully balanced for even intensity.
What is the SDS-PAGE ladder?
This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 30 and 80 kDa bands have quadruple the mass of the other bands and serve as reference indicators. This ladder is pre-mixed with loading buffer and ready to load.
Where do you store a protein ladder?
Review other protein ladders in the unstained and prestained protein ladder guide. This product was previously called Prism Ultra Protein Ladder (10-245 kDa). Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
What is the ladder for Western blot?
Western blot protein ladders are a mixture of pre-stained, StrepTactin-HRP Conjugate proteins (or Strep-tagged recombinant proteins). It enables fluorescent visualization of protein gels and chemiluminescent or colorimetric immunodetection of western blots.
What is a ladder in biology?
A ladder is a solution that contains a series of well-defined DNA fragments of particular lengths. Some ladders may contain DNA fragments of sizes such as 100 base pairs all the way up to 10,000 base pairs (10 kbp).
What is a ladder in bio?
What is the purpose of the protein ladder in Western blot?
Western Blot specific protein ladders are specifically designed for easy and convenient protein molecular weight estimation directly on western blots or indirectly by using various blotting detection systems (e.g. chemiluminescence, fluorescence, or chromogenic).
What are Biobio-Rad protein ladders?
Bio-Rad protein ladders have been formulated and combined for use as molecular weight markers in a variety of applications including SDS-PAGE, IEF, 2-D PAGE, and western blotting. For SDS-PAGE, choose from prestained and unstained, natural or recombinant protein ladders for broad, high, and low molecular weight ranges.
What is a protein ladder for SDS PAGE?
Protein Ladder Selection Bio-Rad protein ladders have been formulated and combined for use as molecular weight markers in a variety of applications including SDS-PAGE, IEF, 2-D PAGE, and western blotting. For SDS-PAGE, choose from prestained and unstained, natural or recombinant protein ladders for broad, high, and low molecular weight ranges.
What is a puregene kit?
Puregene Accessories used in combination with Gentra Puregene Kits enable purification of high-molecular-weight (100–200 kb) DNA suitable for archiving. The scalable purification procedure gently removes contaminants and inhibitors and allows large-volume samples to be purified for use as long-term references.
What is a recombinant protein ladder?
Every recombinant protein ladder is broad range. Prestains and Tags: Proteins within a ladder may be stained all one color, have two or more bands stained different colors to make sure that the molecular weights within the ladder are easily identified, or have a multicolored pattern like Kaleidoscope™ protein standards.