How is lentivirus MOI calculated?
MOI stands for Multiplicity of Infection which refers to the number of viral particles per cell. To calculate, take the number of viral particles used per well then divide by the number of cells originally seeded in the well. This equals the MOI.
What is the optimal MOI?
The optimal MOI for each cell line varies. For each cell line, a range of MOI from 5-100 needs to be tested using a positive control particles containing a fluorescent protein marker, such as OriGene’s GFP control lentiviral particle (cat# PS100071V).
What is a good lentivirus titer?
A: A typical lentiviral titer from crude supernatant is 1×10^6 TU/mL.
How do you test for lentivirus?
The most accurate method of lentiviral titration uses qPCR to measure the number of proviral copies that have integrated into a target cell’s genome. This approach can discriminate multiple integration events, reducing the possibility of underestimating the viral titer.
How do you calculate MOI from GC mL?
For an MOI = 1, the volume (in µl) of AAV particles needed = ((total number of cells per well)/(number of genome copies (GC)/ml)) x 1,000. The MOI used is critical to achieve 100% infection of the target cells without causing major side effects.
How do you calculate MOI from IFU mL?
The MOI is expressed as a ratio of the number of IFU or TU added to the cells (IFU/cell or TU/cell). For example, if your cell line requires an MOI of 200, this would require 200 IFU per 1 cell. LP/mL is a measure of physical titer and will need to be converted to IFU/mL to determine the MOI.
How do you calculate bacteria MOI?
The MOI is determined by simply dividing the number of phage added (ml added x PFU/ml) by the number of bacteria added (ml added x cells/ml).
What is a low MOI?
High MOI is used when the experiment requires that every cell in the culture is infected. By contrast, low MOI is used when multiple cycles of infection are required.
What is PFU per mL?
The pfu/mL result represents the number of infective particles within the sample and is based on the assumption that each plaque formed is representative of one infective virus particle.