Is TPPA and TPHA same?
A modification of the TPHA is the Treponema pallidum particle agglutination assay (TPPA), which has been shown (1) to perform as well as the hemagglutination assay.
What is TPPA syphilis?
The Treponema pallidum particle agglutination assay (also called TPPA test) is an indirect agglutination assay used for detection and titration of antibodies against the causative agent of syphilis, Treponema pallidum subspecies pallidum. It also detects other treponematoses.
What is RPR and TPPA?
EIA = Enzyme Immunoassay, RPR = Rapid Plasma Reagin, TPPA = Treponema pallidum Particle Agglutination.
Is TPPA a confirmatory test?
The TP-PA test is used to confirm the reactive results (2, 3) of a nontreponemal screening test for syphilis, such as the Venereal Disease Research Laboratory (VDRL) slide test, or as a diagnostic test in patients with a nonreactive nontreponemal test but with signs or symptoms suggestive of late syphilis.
Can TPPA test false positive?
These false-positive results are often transient with unknown cause. False-positive results may occur in association with other underlying illnesses. Serodia TP-PA may be reactive in persons from areas endemic for yaws or pinta. Serodia TP-PA performs best in populations at risk for T pallidum infection.
How long is TPPA positive?
The positive test usually goes back to normal in about six months. If a treponemal test (FTA-ABS or TPPA) is positive but a nontreponemal test is negative (RPR and VDRL), this can mean one of two things: Successfully treated syphilis (if the patient remembers being treated)
What does a reactive TPPA mean?
Reactive A reactive T. pallidum antibody initial screen (TPA), with a non-reactive RPR and a reactive second T. pallidum antibody test (TPPA), suggests exposure to syphilis. Persons with a history of previous treatment will require no further treatment unless re-exposure is likely.
What does TPPA negative mean?
If the TPPA is negative then a 2nd EIA is done, which will be different (e.g. a different manufacturer or a different part of the antigen or antibody being detected, see diagram below), or an IgG immunoblot can be used.
What does a positive TPPA mean?
In the setting of a positive syphilis IgG screening result and a negative RPR, a positive TP-PA result is consistent with either 1) past, successfully treated syphilis, 2) early syphilis with undetectable RPR titers, or 3) late/latent syphilis in patients who do not have a history of treatment for syphilis.
Can TP-PA be false-negative?
With TPPA as reference, the POCT had 11 false positive and 145 false negative results, giving a sensitivity of 59.6% (95% confidence interval CI: 54.3-64.7%) and specificity of 99.4% (95% CI: 98.9-99.7%) (Table 1).
What causes a false-positive TP-PA?
Can TPPA be false positive?
Can TPPA test false negative?
Can TP-PA test false-positive?
Can TPPA test false-positive?